morphological and morphometrical changes of endometrim after application of estrogen and progesterone during luteal phase in the superovulated mice

background: the application of luteal phase supplementation hormones to increase the implantation rate is on debate among researchers. objective: in this study, the morphological and morphometrical assessment of superovulated mice endometrium were investigated at window implantation period superovulated mice. materials and methods: female mice were superovualated then were mated with vasectomized mice; the mice were divided in experimental and control groups. experimental group included five groups which in them pseudopregnant mice were given a four consecutive daily injection of 1-progesterone (p), 2-estrogen (e), 3-estrogen + progesterone, 4-antiprogesterone+estrogen and 5-sham group. the uterine of all groups were collected after 4.5 day of pseudopregnancy and were prepared for histological and morphometrical studies. results: morphological studies of endometrial tissue showed that the luminal epithelium in group p appeared cuboidal shape. endometrial folding was very high in group e+p. the luminal epithelium in groups e, e+p and ru 486 + e were seen in different morphology in comparison to control group. morphometrical evaluation also showed height of luminal epithelium in group e (32.7±0.67) and e+p (33.6±1.3) were higher than those were seen in control (22.5±1.5) and group p (15.3±1.2). conclusion: progesterone caused the lowest endometrial development compare to other groups. it is concluded that the adding of e to p may improve endometrial condition to implant at luteal phase.